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Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli

The objective of this study was to evaluate the inhibitory capacity of Lactiplantibacillus plantarum LP5 against Campylobacter coli in in vitro biofilm formation and competitive exclusion assays. Biofilm formation by C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 and C. coli DSPV570 was evaluat...

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Autores Principales: Ruiz, María Julia, Sirini, Noelí Estefanía, Zimmermann, Jorge Alberto, Soto, Lorena Paola, Zbrun, María Victoria, Sequeira, Gabriel Jorge, Olivero, Carolina Raquel, Rosmini, Marcelo Raúl, Signorini, Marcelo Lisandro, Frizzo, Laureano Sebastián
Formato: Artículo revista
Lenguaje:spa
Publicado: Universidad Nacional del Litoral 2022
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11415
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institution Universidad Nacional del Litoral
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language spa
format Artículo revista
description The objective of this study was to evaluate the inhibitory capacity of Lactiplantibacillus plantarum LP5 against Campylobacter coli in in vitro biofilm formation and competitive exclusion assays. Biofilm formation by C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 and C. coli DSPV570 was evaluated by OD measurement. The inhibitory capacity of L. plantarum LP5 against C. coli was evaluated on glass, nylon and aluminium discs, added with L. plantarum and incubated at 37°C for 72 h. C. coli was added to each washed well. The plates were incubated at 42°C for 72 h in microaerophilic conditions and the biofilms were detached for quantification. The four strains of C. coli were classified as moderate biofilm former. The competitive exclusion test showed that the biofilm formation of the C. coli strains in all materials was significantly higher than the biofilm formation of each pathogen in the presence of L. plantarum LP5 biofilms. Although it is necessary to carry out more tests to confirm the ability of C. coli to survive in hostile environments until reaching the host, this study would allow progress in the elucidation of its behaviour through the formation of biofilms.
author Ruiz, María Julia
Sirini, Noelí Estefanía
Zimmermann, Jorge Alberto
Soto, Lorena Paola
Zbrun, María Victoria
Sequeira, Gabriel Jorge
Olivero, Carolina Raquel
Rosmini, Marcelo Raúl
Signorini, Marcelo Lisandro
Frizzo, Laureano Sebastián
spellingShingle Ruiz, María Julia
Sirini, Noelí Estefanía
Zimmermann, Jorge Alberto
Soto, Lorena Paola
Zbrun, María Victoria
Sequeira, Gabriel Jorge
Olivero, Carolina Raquel
Rosmini, Marcelo Raúl
Signorini, Marcelo Lisandro
Frizzo, Laureano Sebastián
Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
author_facet Ruiz, María Julia
Sirini, Noelí Estefanía
Zimmermann, Jorge Alberto
Soto, Lorena Paola
Zbrun, María Victoria
Sequeira, Gabriel Jorge
Olivero, Carolina Raquel
Rosmini, Marcelo Raúl
Signorini, Marcelo Lisandro
Frizzo, Laureano Sebastián
author_sort Ruiz, María Julia
title Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
title_short Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
title_full Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
title_fullStr Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
title_full_unstemmed Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli
title_sort ability of lactiplantibacillus plantarum lp5 to inhibit biofilms of campylobacter coli
publisher Universidad Nacional del Litoral
publishDate 2022
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11415
_version_ 1737441561627590656
spelling oai:bibliotecavirtual.unl.edu.ar:article-114152022-07-01T21:18:48Z Ability of Lactiplantibacillus plantarum LP5 to inhibit biofilms of Campylobacter coli Capacidad de Lactiplantibacillus plantarum LP5 para inhibir biopelículas de Campylobacter coli Ruiz, María Julia Sirini, Noelí Estefanía Zimmermann, Jorge Alberto Soto, Lorena Paola Zbrun, María Victoria Sequeira, Gabriel Jorge Olivero, Carolina Raquel Rosmini, Marcelo Raúl Signorini, Marcelo Lisandro Frizzo, Laureano Sebastián The objective of this study was to evaluate the inhibitory capacity of Lactiplantibacillus plantarum LP5 against Campylobacter coli in in vitro biofilm formation and competitive exclusion assays. Biofilm formation by C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 and C. coli DSPV570 was evaluated by OD measurement. The inhibitory capacity of L. plantarum LP5 against C. coli was evaluated on glass, nylon and aluminium discs, added with L. plantarum and incubated at 37°C for 72 h. C. coli was added to each washed well. The plates were incubated at 42°C for 72 h in microaerophilic conditions and the biofilms were detached for quantification. The four strains of C. coli were classified as moderate biofilm former. The competitive exclusion test showed that the biofilm formation of the C. coli strains in all materials was significantly higher than the biofilm formation of each pathogen in the presence of L. plantarum LP5 biofilms. Although it is necessary to carry out more tests to confirm the ability of C. coli to survive in hostile environments until reaching the host, this study would allow progress in the elucidation of its behaviour through the formation of biofilms. El objetivo de este estudio fue evaluar la capacidad inhibitoria de Lactiplantibacillus plantarum LP5 frente a Campylobacter coli en ensayos de formación de biopelículas in vitro y exclusión competitiva. La formación de biopelículas por C. coli NCTC11366, C. coli DSPV458, C. coli DSPV541 y C. coli DSPV570 fue evaluada mediante medición de DO. La capacidad inhibitoria de L. plantarum LP5 frente a C. coli fue evaluada sobre discos de vidrio, nailon y aluminio. Sobre una biopelícula de L. plantarum se adicionó C. coli para cuantificar el efecto inhibidor de L. plantarum LP5 sobre el patógeno. Las cuatro cepas de C. coli fueron clasificadas como moderadas formadoras de biopelículas. El ensayo de exclusión competitiva mostró que la formación de biopelículas de las cepas de C. coli en todos los materiales fue significativamente mayor que la formación de biopelículas de cada patógeno en presencia de biopelículas de L. plantarum LP5. Si bien es necesario realizar más pruebas para confirmar la capacidad de supervivencia de C. coli en ambientes hostiles hasta llegar al huésped, este estudio permitiría avanzar en el esclarecimiento de su comportamiento mediante la formación de biopelículas.  Universidad Nacional del Litoral 2022-04-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo Articulo info:ar-repo/semantics/artículo Artículo original Original article application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11415 10.14409/favecv.2022.1.e0002 FAVE Sección Ciencias Veterinarias; Vol. 21 (2022): FAVE Sección Ciencias Veterinarias; e0002 FAVE Sección Ciencias Veterinarias; Vol 21 (2022): FAVE Sección Ciencias Veterinarias; e0002 FAVE Sección Ciencias Veterinarias; v. 21 (2022): FAVE Sección Ciencias Veterinarias; e0002 2362-5589 1666-938X 10.14409/favecv.2022.1 spa info:eu-repo/grantAgreement/EC/FP7/ https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11415/15666 /*ref*/Cáceres ME, Etcheverría AI, Padola NL. 2019. Efectos del medio de cultivo y de la metodología aplicada sobre la formación de biopelículas de 2 cepas de Escherichia coli diarreagénicas. Rev. Argent. Microbiol. 51: 208-213. /*ref*/Camargo AC, Woodward JJ, Call DR, Nero LA. 2017. Listeria monocytogenes in food-processing facilities, food contamination, and human listeriosis: the Brazilian scenario. Foodborne Pathog. Dis. 14: 623-636. /*ref*/Castellano P, Belfiore C, Fadda S, Vignolo G. 2008. A review of bacteriocinogenic lactic acid bacteria used as bioprotective cultures in fresh meat produced in Argentina. Meat. Sci. 79: 483-499. /*ref*/Gómez NC, Ramiro JM, Quecan BX, de Melo Franco BD. 2016. Use of potential probiotic lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes, Salmonella Typhimurium, and Escherichia coli O157: H7 biofilms formation. Front. Microbiol. 7: 863. /*ref*/Guerrieri E, de Niederhäusern S, Messi P, Sabia C, Iseppi R, Anacarso I, Bondi M. 2009. Use of lactic acid bacteria (LAB) biofilms for the control of Listeria monocytogenes in a small-scale model. Food Control. 20: 861-865. /*ref*/Gunther IV NW, Chen CY. 2009. The biofilm forming potential of bacterial species in the genus Campylobacter. Food Microbiol. 26: 44-51. /*ref*/Kalmokoff M, Lanthier P, Tremblay TL, Foss M, Lau PC, Sanders G, Szymanski CM. 2006. Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation. J. Bacteriol. 188: 4312-4320. /*ref*/Monteiro GP, Rossi DA, Valadares EC, Peres PAB, Braz RF, Notário FO, Fonseca BB. 2021. Lactic bacterium and Bacillus sp. biofilms can decrease the viability of Salmonella gallinarum, Salmonella heidelberg, Campylobacter jejuni and methicillin resistant Staphylococcus aureus on different substrates. Rev. Bras. Cienc. Avic. 23: 1-12. /*ref*/Morales P, Aguirre J, Troncoso M, Figueroa G. 2020. Comparison of in vitro and in situ antagonism assays as tools for the selection of bio-preservative lactic acid bacteria (LAB) in poultry meat. LWT. 118: 108846. /*ref*/Reeser RJ, Medler RT, Billington SJ, Jost BH, Joens LA. 2007. Characterization of Campylobacter jejuni biofilms under defined growth conditions. Appl. Environ. Microbiol. 73: 1908-1913. /*ref*/Rossler E, Fuhr EM, Lorenzón G, Romero-Scharpen A, Berisvil AP, Blajman JE, Astesana DM, Zimmermann JA, Fusari ML, Signorini ML, Soto LP, Frizzo LS, Zbrun MV. 2017. Presencia de serotipos de Campylobacter jejuni O:19 en la cadena avícola Argentina. Rev. Argent. Microbiol. 49: 178-182. /*ref*/Ruiz MJ. 2019. Aislamiento e identificación de bacterias ácido lácticas con actividad inhibitoria de bacterias implicadas en enfermedades transmitidas por alimentos. Tesis doctoral, FCV-UNICEN. 124 pp. /*ref*/Ruiz MJ, Zbrun MV, Signorini ML, Zimmermann JA, Soto LP, Rosmini MR, Frizzo LS. 2021. In vitro screening and in vivo colonization pilot model of Lactobacillus plantarum LP5 and Campylobacter coli DSPV 458 in mice. Arch. Microbiol. 1-11. /*ref*/Shokri D, Khorasgani MR, Mohkam M, Fatemi SM, Ghasemi Y, Taheri-Kafrani A. 2018. The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics Antimicrob. Proteins. 10: 34-42. info:eu-repo/semantics/openAccess Derechos de autor 2022 Noelí Estefanía Sirini, Jorge Alberto Zimmermann http://creativecommons.org/licenses/by-nc-sa/4.0
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