Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes
The interaction of the anti-inflammatory drugs diclofenac and flunixin on the pharmacokinetic behavior of enrofloxacin and its active metabolite, ciprofloxacin, was studied in calves. Control group (n=10) received 5 mg/kg of enrofloxacin intramuscularly. Two weeks later the treatment was repeated an...
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Universidad Nacional del Litoral
2022
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| Acceso en línea: | https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11543 |
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oai:bibliotecavirtual.unl.edu.ar:article-115432022-06-24T21:45:46Z Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes Urzúa, N The interaction of the anti-inflammatory drugs diclofenac and flunixin on the pharmacokinetic behavior of enrofloxacin and its active metabolite, ciprofloxacin, was studied in calves. Control group (n=10) received 5 mg/kg of enrofloxacin intramuscularly. Two weeks later the treatment was repeated and calves from group A (n=5) received 2.2 mg/kg of flunixin while group B (n=5) 1 mg/kg of diclofenac, 2 weeks later the treatments were exchanged. Heparinized blood samples were obtained at different times after application. Plasma was treated with water, homogenizing solution, and difloxacin as an internal standard. The whole was centrifuged and 50 μL of the supernatant was injected to quantify enrofloxacin and ciprofloxacin by HPLC coupled to a fluorescence detector at 295 nm excitation and 490 nm emission. Plasma concentrations vs. time of enrofloxacin and ciprofloxacin per animal were analyzed by the PK Solution 2.0 program. Intramuscular application of enrofloxacin shows rapid absorption and moderate plasma permanence. Diclofenac significantly increases Cmáx and area under the curve, while flunixin does not affect enrofloxacin kinetics. Enrofloxacin with flunixin or diclofenac co-administration modifies enrofloxacin into ciprofloxacin transformation, results that may influence the therapeutic design. Se estudió la interacción de los antiinflamatorios diclofenac y flunixin sobre el comportamiento farmacocinético de enrofloxacina y su metabolito activo, ciprofloxacina en terneros. El grupo control (n=10) recibió 5 mg/kg de enrofloxacina por vía intramuscular. Dos semanas después se repitió el tratamiento y terneros del grupo A (n=5) recibieron 2.2 mg/kg de flunixin mientras el grupo B (n=5) 1 mg/kg de diclofenac, 2 semanas después se intercambiaron los tratamientos. En distintos tiempos post aplicación se obtuvieron muestras de sangre heparinizada. El plasma fue tratado con agua, una solución homogeneizadora y difloxacina como estándar interno. El conjunto se centrifugó y 50 μL del sobrenadante se inyectaron para cuantificar enrofloxacina y ciprofloxacina por HPLC acoplado a detector de fluorescencia a 295 nm de excitación y 490 nm de emisión. Las concentraciones plasmáticas vs tiempo por animal se analizaron por el programa PK Solution 2.0. La aplicación intramuscular de enrofloxacina demuestra rápida absorción y moderada permanencia plasmática. Diclofenac aumenta significativamente el Cmáx y el área bajo la curva mientras flunixin no afecta la cinética de enrofloxacina y la co-administración de enrofloxacina con flunixin o diclofenac modifica la transformación de enrofloxacina a ciprofloxacina, resultados que pueden influenciar el diseño terapéutico. Universidad Nacional del Litoral 2022-05-24 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo Articulo info:ar-repo/semantics/artículo application/pdf https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11543 FAVE Sección Ciencias Veterinarias; Vol. 20 Núm. Suplemento (2022): Resúmenes Jornadas FCV-UNL 2020 FAVE Sección Ciencias Veterinarias; Vol 20 No Suplemento (2022): Resúmenes Jornadas FCV-UNL 2020 FAVE Sección Ciencias Veterinarias; v. 20 n. Suplemento (2022): Resúmenes Jornadas FCV-UNL 2020 2362-5589 1666-938X 10.14409/favecv.v20iSuppl2. spa info:eu-repo/grantAgreement/EC/FP7/ https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11543/15765 info:eu-repo/semantics/openAccess Derechos de autor 2022 Urzua http://creativecommons.org/licenses/by-nc-sa/4.0 |
| institution |
Universidad Nacional del Litoral |
| collection |
Publicaciones periódicas |
| language |
spa |
| format |
Artículo revista |
| description |
The interaction of the anti-inflammatory drugs diclofenac and flunixin on the pharmacokinetic behavior of enrofloxacin and its active metabolite, ciprofloxacin, was studied in calves. Control group (n=10) received 5 mg/kg of enrofloxacin intramuscularly. Two weeks later the treatment was repeated and calves from group A (n=5) received 2.2 mg/kg of flunixin while group B (n=5) 1 mg/kg of diclofenac, 2 weeks later the treatments were exchanged. Heparinized blood samples were obtained at different times after application. Plasma was treated with water, homogenizing solution, and difloxacin as an internal standard. The whole was centrifuged and 50 μL of the supernatant was injected to quantify enrofloxacin and ciprofloxacin by HPLC coupled to a fluorescence detector at 295 nm excitation and 490 nm emission. Plasma concentrations vs. time of enrofloxacin and ciprofloxacin per animal were analyzed by the PK Solution 2.0 program. Intramuscular application of enrofloxacin shows rapid absorption and moderate plasma permanence. Diclofenac significantly increases Cmáx and area under the curve, while flunixin does not affect enrofloxacin kinetics. Enrofloxacin with flunixin or diclofenac co-administration modifies enrofloxacin into ciprofloxacin transformation, results that may influence the therapeutic design. |
| author |
Urzúa, N |
| spellingShingle |
Urzúa, N Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| author_facet |
Urzúa, N |
| author_sort |
Urzúa, N |
| title |
Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| title_short |
Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| title_full |
Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| title_fullStr |
Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| title_full_unstemmed |
Interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| title_sort |
interacción de diclofenac y flunixín en el comportamiento farmacocinético de enrofloxacina en terneros lactantes |
| publisher |
Universidad Nacional del Litoral |
| publishDate |
2022 |
| url |
https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/11543 |
| _version_ |
1736973468584378368 |
| score |
11.8626 |