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EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus

Rg1 ginsenoside, the main active compound of Panax ginseng extract, could be a promising vaccine adjuvant against Staphylococcus aureusbovine mastitis. The aim was to evaluate the adjuvant effects of Rg1 combined with liposomes or alone against S. aureus lysate from bovine mastitis in mice. Female B...

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Autor Principal: Silvestrini, P
Formato: Artículo revista
Publicado: Universidad Nacional del Litoral 2021
Acceso en línea:https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10910
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spelling oai:bibliotecavirtual.unl.edu.ar:article-109102021-12-03T13:14:27Z EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus EVALUATION OF ADJUVANT CAPACITY OF GINSENOSIDE RG1 IN BALB/C MICE IMMUNIZED WITH A Staphylococcus aureus LYSATE Silvestrini, P Rg1 ginsenoside, the main active compound of Panax ginseng extract, could be a promising vaccine adjuvant against Staphylococcus aureusbovine mastitis. The aim was to evaluate the adjuvant effects of Rg1 combined with liposomes or alone against S. aureus lysate from bovine mastitis in mice. Female Balb/c mice were divided into 4 groups: S. aureus Lysate + IFA (Group I), S. aureus lysate + liposomes (Group II), S. aureus lysate + Rg1 (Group III) and S. aureus lysate + liposomes + Rg1 (Group IV). All mice were subcutaneously immunized with all formulations 3 times at 2-weeks intervals. Humoral response was evaluated by indirect IgG ELISA assay in plasma collected at the end of the assay. Cellular response was evaluated by MTT kit assay in spleen cells from immunized mice. Groups I and IV showed higher IgG values than groups II and III (p < 0.05). Groups I and II showed higher proliferation values than groups III and IV (p < 0.05). The Rg1-liposome combination increased S. aureus-specific IgG production. However, Rg1 was not able to stimulate lymphocyte proliferation from immunized mice, possibly due to the short S. aureus-specific stimulation time. New assays with different experimental conditions are needed to elucidate adjuvant effects of Rg1 in a murine model. Universidad Nacional del Litoral 2021-12-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion artículo Articulo info:ar-repo/semantics/artículo https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10910 10.14409/favecv.v19isuplemento.10910 FAVE Sección Ciencias Veterinarias; Vol. 19 Núm. suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; Vol 19 No suplemento (2021): FAVE Sección Ciencias Veterinarias FAVE Sección Ciencias Veterinarias; v. 19 n. suplemento (2021): FAVE Sección Ciencias Veterinarias 2362-5589 1666-938X 10.14409/favecv.v19isuplemento info:eu-repo/grantAgreement/EC/FP7/ info:eu-repo/semantics/openAccess Derechos de autor 2021 P Silvestrini http://creativecommons.org/licenses/by-nc-sa/4.0
institution Universidad Nacional del Litoral
collection Publicaciones periódicas
format Artículo revista
description Rg1 ginsenoside, the main active compound of Panax ginseng extract, could be a promising vaccine adjuvant against Staphylococcus aureusbovine mastitis. The aim was to evaluate the adjuvant effects of Rg1 combined with liposomes or alone against S. aureus lysate from bovine mastitis in mice. Female Balb/c mice were divided into 4 groups: S. aureus Lysate + IFA (Group I), S. aureus lysate + liposomes (Group II), S. aureus lysate + Rg1 (Group III) and S. aureus lysate + liposomes + Rg1 (Group IV). All mice were subcutaneously immunized with all formulations 3 times at 2-weeks intervals. Humoral response was evaluated by indirect IgG ELISA assay in plasma collected at the end of the assay. Cellular response was evaluated by MTT kit assay in spleen cells from immunized mice. Groups I and IV showed higher IgG values than groups II and III (p < 0.05). Groups I and II showed higher proliferation values than groups III and IV (p < 0.05). The Rg1-liposome combination increased S. aureus-specific IgG production. However, Rg1 was not able to stimulate lymphocyte proliferation from immunized mice, possibly due to the short S. aureus-specific stimulation time. New assays with different experimental conditions are needed to elucidate adjuvant effects of Rg1 in a murine model.
author Silvestrini, P
spellingShingle Silvestrini, P
EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
author_facet Silvestrini, P
author_sort Silvestrini, P
title EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
title_short EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
title_full EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
title_fullStr EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
title_full_unstemmed EVALUACIÓN DE LA CAPACIDAD ADYUVANTE DEL GINSENÓSIDO RG1 EN RATONES BALB/C INMUNIZADOS CON UN LISADO DE Staphylococcus aureus
title_sort evaluación de la capacidad adyuvante del ginsenósido rg1 en ratones balb/c inmunizados con un lisado de staphylococcus aureus
publisher Universidad Nacional del Litoral
publishDate 2021
url https://bibliotecavirtual.unl.edu.ar/publicaciones/index.php/FAVEveterinaria/article/view/10910
_version_ 1718763518893752320
score 11.8626

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